Topics in Depth
The Theme of Common Mistakes in Biotechnology
DNA will migrate to the positive electrode due to its negatively charged backbone. This is usually the red colored electrode, so remember, "run to red."
Electro this, electro that, electro what? Both electrophoresis and electroporation use electricity or a current to do their jobs. Electrophoresis separates nucleic acids based on size and charge and electroporation creates pores so DNA can enter a cell.
With the compass naming system, it's easy to forget exactly what a Southern, Northern and Western blot actually look for and how you would choose which technique to use. Just remember this: Sally Donned Nancy's Meticulous White Pants (Southern = DNA, Northern = mRNA, Western = protein).
You might think that only the anti-sense is necessary for RNA interference, but, in fact, the buddy system is strictly enforced in RNAi. Double-stranded siRNA is essential for RNAi to occur.
Differentiated or dedifferentiated? Struggling to understand these terms that sound so much alike? Remember that in order to become dedifferentiated, cells have to be differentiated first. The prefix "de-" means something has been taken away.
Forensic scientists do not actually look at the differences in the sequence of nucleotides between the DNA left at a crime scene and the suspects. They analyze the number of repeats of small DNA sequences.
Genes cannot be delivered to human cells alone in gene therapy. They first have to be cloned into an efficient vehicle first. We're not talking about a Toyota Prius here, but a viral vector that can deliver the gene to the target cells and incorporate the gene into their genome.
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