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Biotechnology: Don't Shoot the Messenger (RNA) True or False

1. Digestion of DNA and a cloning vector with a restriction enzyme usually generates products with -> Blunt ends
2. Chain termination is achieved in dideoxy DNA sequencing by -> Incorporation of a dideoxy dNTP
3. Only a small amount of sample is needed for PCR because -> PCR amplifies the amount of starting material
4. A scientist would use RFLP analysis to look for the difference in -> None of the above
5. Which of the following terms does not belong? Or, if you never stopped loving Sesame Street, "one of these things is not like the others…" -> DNA probe
6. Although it is much faster than creating a knock out organism, which of the following is a disadvantage of using RNAi? -> It requires large amounts of siRNA
7. Embryonic stem cells are desirable for research use because they are -> Differentiated
8. In animal cloning, the nucleus of a differentiated cell is transferred into which of the following type of cell? -> Enucleated egg cell
9. Only a small sample is needed when using DNA as evidence because -> None of the above
10. The type of vector typically used in gene therapy is a(n) -> Bacterial vector

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