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Biotechnology: When a Single Helix Just Won't Do True or False

1. Restriction fragments can be separated from each other based on size using -> Restriction enzyme digestion
2. How can a scientist ensure that all of the bacteria they are growing contain the recombinant DNA they have introduced? -> Incubate the bacteria with the same antibiotic for which the recombinant DNA expresses a resistance gene
3. Why do cloning vectors contain genes for antibiotic resistance in addition to target genes? -> The bacteria are toxic to plants and have to be killed before the genes can be transferred to plants
4. DNA microarray analysis would be useful in which of the following situations? -> To identify a particular DNA mutation
5. The step of PCR that has to occur prior to primers binding to the DNA is -> Denaturation
6. RNAi requires which of the following? -> Primers
7. A farmer has a crop of papaya that are being killed by a virus. How could genetic modification of the papaya plants be beneficial? -> Viruses that infect papaya will not grow in genetically modified plants
8. How are seedless watermelons, which are modified using chemicals, a type of genetic modification? -> Seeds are treated with chemicals creating a tetraploid plant that is crossed with a diploid plant, creating a sterile triploid
9. A condition that is caused by a gene of known sequence can be diagnosed by -> PCR analysis
10. Which of the following is not a problem associated with gene therapy? -> Cloning a gene into a viral vector is met with little success