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Biotechnology

Biotechnology

Biotechnology: When a Single Helix Just Won't Do Quiz

Think you’ve got your head wrapped around Biotechnology? Put your knowledge to the test. Good luck — the Stickman is counting on you!
Q. Restriction fragments can be separated from each other based on size using


Electrophoresis
Restriction enzyme digestion
PCR
RT-PCR
Southern blotting
Q. How can a scientist ensure that all of the bacteria they are growing contain the recombinant DNA they have introduced?


Incubate the bacteria at a high temperature
Incubate the bacteria at a low temperature
Incubate the bacteria with the same antibiotic for which the recombinant DNA expresses a resistance gene
Incubate the bacteria for a short period of time
Incubate the bacteria for a long period of time
Q. Which of the following terms is out of place?


PCR
Denaturation
In situ hybridization
Annealing
Extension
Q. DNA microarray analysis would be useful in which of the following situations?


To determine the length of a DNA fragment
To determine the sequence of a piece of DNA
To identify a particular DNA sequence
To identify a particular DNA mutation
To look at global changes in gene expression
Q. The step of PCR that has to occur prior to primers binding to the DNA is


Denaturation
Annealing
Extension
Propagation
Filtration
Q. RNAi requires which of the following?


Primers
DNA probes
RNA polymerase
siRNAs
siDNAs
Q. The first plant that was cloned from a single cell was a


Tomato plant
Carrot plant
Pea plant
Bean plant
Corn plant
Q. The first mammal to be cloned using the nucleus from a differentiated cell was a(n)


whale
cow
pig
aardvark
sheep
Q. A condition that is caused by a gene of known sequence can be diagnosed by


DNA microarray analysis
Western blotting
In vitro mutagenesis
PCR analysis
All of the above
Q. Which of the following is not a problem associated with gene therapy?


Cloning a gene into a viral vector is met with little success
The effect is not long lasting
Gene therapy may activate an immune response
Diseases can be the result of more than one gene
Viral vectors can be toxic to human cells
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