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Biotechnology

Biotechnology

Biotechnology Terms

Get down with the lingo

Western Blot

Technique used to identify proteins. Proteins are separated by electrophoresis and then transferred to a nitrocellulose membrane. The membrane is incubated with an antibody specific for a particular protein. Then a secondary antibody specific for the organism that produced the first (primary) antibody is added. The secondary antibody is involved in a chemical reaction that produces a product that can be visualized. It's a cousin of the Northern and Southern blots.

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