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DNA Structure, Replication, and Technology

DNA Structure, Replication, and Technology

DNA Structure, Replication, and Technology: Totally Phased True or False

1. We never talked about other enzymes that modify histones affecting packaging of genes. This leads to inhibition of gene expression or activation of gene expression, depending on how open DNA packaging is, where more packaging = gene repression. Can you fill in the table below knowing the information that you know? The answer is "yes". (answers in bold) Which of the following enzymes is MOST likely to be responsible for adding acetyl groups to histones? -> Histone methyltransferase
2. Which of the following enzymes is most likely to increase gene expression? -> An enzyme that adds a negative phosphate group to histones
3. What is the likely function of arginine deiminase? -> Adds an arginine to DNA
4. Given the picture (A) above, in which stage of the cell cycle is the cell shown? -> Interphase
5.

Given the picture (B) above, in which stage of the cell cycle is the cell shown? -> Prophase

6.

 Given the picture (C) above, in which stage of the cell cycle is the cell shown? -> Telophase/Cytokinesis

7.

Given the picture (M) above, in which stage of the cell cycle is the cell shown? -> Telophase/Cytokinesis

8. Which of the following would be the best way to insert a PCR product into a vector? -> All of the above would be good strategies.
9. Which of the following is only found in RNA? -> 5´-CUUGGAUCCGGUACGU-3´
10. Which of the following would be the best way to genetically modify a corn plant that carries a gene that is >20 kb in length? -> Put the gene in a cosmid with Ti sequences, and then transform with Agrobacterium.

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