Innocence is closely linked to youth, idealism, and – to a lesser extent – virginity. In this novel, reference to the protagonist’s innocence almost always evokes nature imagery, specifically the season of spring full of flowers and blossoms. Innocence, then, is considered a "natural" state, the condition in which God first situates humanity. Early in the novel, there is an implication that innocence and womanhood cannot exist simultaneously. However, as the narrative unfolds, the protagonist manages to do just that. The loss of innocence has as much to do with the destruction of dreams as with loss of virginity. Interestingly, the loss of innocence inevitably leads to the development of the capacity for deception – creating an interiority and externality in the character that was not present before. Innocence can also be regained – to some extent – through true love, which can restore a person’s youth.
Despite Janie’s hard life and sexual experience, she never completely loses her innocence; her relationship with Tea Cake, in particular, revives her inherent sense of easy trust and wide-eyed wonder.
The crucial scene of the bee and the pear tree can be read Biblically, with Janie’s backyard as the Garden of Eden and Janie as Eve.