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Microorganisms: Viruses

Microorganisms: Viruses

Microorganisms: Viruses: Shmoopvirus is Infectious True or False

1. Which of the following is NOT involved in any type of virus membrane fusion?Cell receptor
2. If you treat a cell with a membrane-impermeable protease and then add a virus to it, the cell will not be infected. Why not?The protease removes the virus receptor from the cell.
3. What does the previous answer tell you about the virus?The virus requires a cellular receptor to enter the cell.
4. How can you prove that a virus does NOT require low pH to enter a cell?Mix infected cells with uninfected cells
5. Reoviruses will still infect cells if treated with SDS, a harsh detergent that dissolves lipid bilayers. What does this information tell you about reoviruses?Reoviruses do not have an envelope.
6. If you infect cells with influenza virus, you can collect virus from the cells in 6 hours, but no cell death is seen. What does this information tell you?The strain is H1N1.
7. A team of scientists have found that the site of replication of Shmoopvirus is in the cytoplasm. What does this tell you about the genes encoded in the Shmoopvirus genome?Shmoopvirus is an enveloped virus.
8. Which of the following viruses would be LEAST affected if you knocked out the host RNA polymerase genes?dsDNA virus
9. Which of the following viruses would be the MOST affected if you knocked out the host DNA polymerase genes?None would be affected
10. If you counted 127 plaques in a cell culture dish after you diluted the infected sample 1:100,000 and added 0.1 mL to cells, what is the virus titre?2.54 × 108 pfu/mL

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