Molecular Genetics: DNA Technology

Transcript

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different ways But but walk us through the techniques like

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how do we how do we manage the expression of

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james this way How do we use dna technology Yes

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The one you want really important use of these sort

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of dna arnie tools is to figure out how gene

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expression changes in different cells and there's a bunch of

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different ways we can do that One is through an

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experiment more than block which basically involves doing electrophoresis on

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already separating the ornate by size and then probing with

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a radioactive probe snippet of dna that tells us Okay

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well we see our jean here and not here basically

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telling us the mara is president in this condition and

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not another condition So that's one way of measuring the

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depression And why would that be useful So let's say

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that you had your comporting myself cancer cells and you

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want to try to find a target that was increased

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in expression in cancer cells You could do it that

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way It's gotta be a drug target Okay that makes

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sense Okay What's rt pcr Yeah Rt pcr So that

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stands for reverse transcription followed by polymerase chain reaction So

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yes kind Of mouthfuls three times the archie step The

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reverse transcription basically means turning already back into today So

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that kind of backwards from we learned before usually dna

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get started already But there's n there enzymes called reverse

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transcriptase is they can do the reverse No i thought

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we couldn't parts in the reverse order They could only

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go in one direction is special different And i think

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that's normal Many memories can't do that But viruses like

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hiv have a special inside and called reverse transcriptase unable

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able to do that Okay All right What is Sit

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two hybridization So inside you have realization yet so that's

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basically a way of looking at where our days are

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located inside of cells So rather than just looking at

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expression levels you can see kind of locations of the

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arnie's as well Cool Okay another five dollars worth dna

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Micro array Yep So it's danny michael wright is kind

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of a more advanced way of looking at um gene

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expression So it's a little chip that you put little

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teeny pieces of dna sticking off of that are kind

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of the probes And then you basically can hybridize your

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aura or some some dna version of your army onto

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that chip And wherever it sticks that tells you oh

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that particular arnie was present in the cell was just

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another way to kind like a northern block but kind

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of a more massive scale God and how does that

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relate With r n a Sequencing Johnny was secreting of

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kind of the most recent version Instead of having to

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use specific probes you basically see what all our day

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in a cell and you're able to do that quantitatively

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so you can say oh there's more of this than

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something else Got it Okay How do we use dna

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technology What is rt pcr What is insight to hybridization 00:03:23.508 --> [endTime] What is it Dna micro array