Molecular Genetics: RNA Interference

Transcript

00:10

A la Shmoop

00:12

We're talking biology with Dr. Ruth Tennen [David introduces Dr. Ruth Tennen]

00:15

and we're gonna talk now about biotechnology.

00:19

Okay, what is RNA interference? [Blood cells separate and the question appears on the screen]

00:22

So, RNA interference, or RNAi,

00:24

is a way of regulating or turning down

00:27

gene expression for specific genes

00:29

using double stranded RNA. [Dr. Ruth Tennen explains gene expressions using double stranded RNA]

00:34

Give us an example of a gene expression

00:36

you'd wanna turn down.

00:38

Okay, so let's say you had --

00:41

I'm gonna use a cancer example again.

00:43

Let's say you had a gene that you thought was required [A segment of RNA highlights]

00:45

for a cancer cell to be alive.

00:47

You could use RNA interference to turn off that gene, basically,

00:50

and see if the cancer cell was still able to survive.

00:53

[ shrieking ] [The bomb explodes and cancer cell is destroyed]

00:54

So, like the cancer cell lived off a certain type of carbon

00:58

- Sure. - or a certain type of whatever.

01:00

It would be something that would be like

01:01

- resistant to that carbon. - Mm-hmm. [David shows various hand gestures]

01:04

Yeah, and the cool thing about RNA interference --

01:06

Because you're not actually messing up the DNA, [DNA and RNA strands]

01:08

you're just messing up the RNA,

01:09

if you get rid of the, kind of, inducer,

01:12

if you get rid of the double-stranded RNA, [RNA strand drops down]

01:14

you can turn the gene back on.

01:15

So it's kind of a reversible or temporary change,

01:17

rather than getting rid of it.

01:19

Got it. Okay. Very cool.

01:21

[ whoop ]

01:22

What is RNA interference? [Questions on RNA Interference and gene expression appears on the screen]

01:25

What's an example of a gene expression you'd wanna turn down?

01:31

[ shrieking ]