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Molecular Genetics: DNA Technology 180 Views
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Transcript
- 00:00
Thank you We sneak in Molecular genetics dna technology Allah
- 00:11
shmoop okay we're on next lesson Dna technology with dr
- 00:17
ruth tenant uh walk us through the various tools and
- 00:20
techniques that people used because it seems like dna technology
- 00:25
is beginning to quote play god in a lot of
Full Transcript
- 00:27
different ways But but walk us through the techniques like
- 00:33
how do we how do we manage the expression of
- 00:36
james this way How do we use dna technology Yes
- 00:41
The one you want really important use of these sort
- 00:43
of dna arnie tools is to figure out how gene
- 00:45
expression changes in different cells and there's a bunch of
- 00:48
different ways we can do that One is through an
- 00:51
experiment more than block which basically involves doing electrophoresis on
- 00:55
already separating the ornate by size and then probing with
- 00:58
a radioactive probe snippet of dna that tells us Okay
- 01:02
well we see our jean here and not here basically
- 01:04
telling us the mara is president in this condition and
- 01:06
not another condition So that's one way of measuring the
- 01:10
depression And why would that be useful So let's say
- 01:14
that you had your comporting myself cancer cells and you
- 01:17
want to try to find a target that was increased
- 01:20
in expression in cancer cells You could do it that
- 01:22
way It's gotta be a drug target Okay that makes
- 01:25
sense Okay What's rt pcr Yeah Rt pcr So that
- 01:30
stands for reverse transcription followed by polymerase chain reaction So
- 01:36
yes kind Of mouthfuls three times the archie step The
- 01:39
reverse transcription basically means turning already back into today So
- 01:43
that kind of backwards from we learned before usually dna
- 01:45
get started already But there's n there enzymes called reverse
- 01:48
transcriptase is they can do the reverse No i thought
- 01:52
we couldn't parts in the reverse order They could only
- 01:55
go in one direction is special different And i think
- 01:57
that's normal Many memories can't do that But viruses like
- 02:01
hiv have a special inside and called reverse transcriptase unable
- 02:05
able to do that Okay All right What is Sit
- 02:08
two hybridization So inside you have realization yet so that's
- 02:13
basically a way of looking at where our days are
- 02:16
located inside of cells So rather than just looking at
- 02:19
expression levels you can see kind of locations of the
- 02:22
arnie's as well Cool Okay another five dollars worth dna
- 02:27
Micro array Yep So it's danny michael wright is kind
- 02:31
of a more advanced way of looking at um gene
- 02:34
expression So it's a little chip that you put little
- 02:37
teeny pieces of dna sticking off of that are kind
- 02:40
of the probes And then you basically can hybridize your
- 02:43
aura or some some dna version of your army onto
- 02:46
that chip And wherever it sticks that tells you oh
- 02:48
that particular arnie was present in the cell was just
- 02:51
another way to kind like a northern block but kind
- 02:53
of a more massive scale God and how does that
- 02:56
relate With r n a Sequencing Johnny was secreting of
- 02:59
kind of the most recent version Instead of having to
- 03:02
use specific probes you basically see what all our day
- 03:04
in a cell and you're able to do that quantitatively
- 03:06
so you can say oh there's more of this than
- 03:07
something else Got it Okay How do we use dna
- 03:13
technology What is rt pcr What is insight to hybridization 00:03:23.508 --> [endTime] What is it Dna micro array